His first appearance is in asdfmovie7 in a scene where Mr. Muffin asks a man called Joey to eat him, but Joey politely refuses. Next, he asked another man to eat him but he is stuffed. In his last appearance in asdfmovie7, the scene turns to a coloured scene and among the panicking of the human characters in the scene, Mr. Muffin jumps up and asks, "Who wants a muffin?".
In asdfmovie8, there is a scene where a man asks "What time is it?". Mr. Muffin comes in with an over the top opening, saying "It's muffin time!". Unfortunately, he is reminded that it is actually 12:30. Finally just giving up, Mr. Muffin shouts "SOMEBODY KILL ME!".
Mr. Muffin makes an appearance in TomSka's music video "THE MUFFIN SONG", making this his first major appearance.
- There is a song that is called It's Muffin Time! on YouTube and samples of Mr. Muffin & Skateboard Cow's appearances on asdfmovie 8
- Mr. Muffin has been confirmed for asdfmovie10, but never made it into the final film.
- Mr. Muffin is shown to be colorful and 3-Dimensional in the movie.
- Once a comment by TomSka says 2 lines of a song about Mr. Muffin, referencing a future music video.
- The 2 Lines "Are Hey have you heard of the suicidal muffin? He says he wants to die even though he's a cute as a button.". Then TomSka says "Oops wrong song" and cuts off the rest of it.
- Mr. Muffin wants to die by letting someone eat him is a reference to how Muffins are eaten by people.
- Mr. Muffin makes his first major appearance in TomSka's music video "THE MUFFIN SONG"