His first appearance is in asdfmovie7 in a scene where Mr. Muffin asks a man called Joey to eat him, but Joey refuses. Next, he asked a fat man to eat him but the fat man is stuffed. In his last appearance in asdfmovie7, the scene turns to a coloured scene and among the panicking of the human characters in the scene, Mr. Muffin jumps up and asks, "Who wants a muffin?".
In asdfmovie8, there is a scene where a man asks "What time is it?". Mr. Muffin comes in with an over the top opening, saying "It's muffin time!". Unfortunately, he is reminded that it is actually 12:30. Finally just giving up, Mr. Muffin shouts "SOMEBODY KILL ME!".
He also appeared in Everybody Do The Flop song doing the flop with Desmond the Moon Bear and the Potato.
- There is a song that is called It's Muffin Time! on youtube and samples of Mr. Muffin & Skateboard Cow's Apperances on asdfmovie 8
- Mr. Muffin has been confirmed for asdfmovie10, but never made it into the final film.
- Mr. Muffin is shown to be colorful and 3-Dimensional in the movie.
- Once a comment by TomSka says 2 lines of a song about Mr.Muffin but since Mr.Muffin dosen't have a song ( It's Muffin Time isn't by TomSka ) These 2 lines could be a preview of a song TomSka is making
- The 2 Lines Are Hey have you heard of the suicidal muffin he says he wants to die even though he's a button then TomSka says Oops wrong song and cuts off the rest of the song.
- Mr. Muffin wants to die by letting someone eat him is a reference to how Muffins are eaten by people.